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KMID : 0545120070170040677
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 4 p.677 ~ p.680
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Bacterial Surface Display of GFPUV on Bacillus subtilis Spores
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Kim June-Hyung
Roh Chang-Hyun
Lee Chang-Won
Kyung Do-Hyun
Choi Soo-Keun
Jung Heung-Chae
Pan Jae-Gu
Kim Byung-Gee
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Abstract
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To analyze a cotG-based Bacillus subtilis spore display system directly, GFPUV was expressed on the surface of Bacillus subtilis spores. When GFPUV was fused to the Cterminal of the cotG structural gene and expressed, the existence of a CotG-GFPUV fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This GFPUV displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli
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KEYWORD
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Bacillus subtilis, cotG, GFPUV, spore, surface
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